Flow Cytometry Competency, Operations, and Quality
 
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Resources

Resources

FlowHubTM is a proprietary resource catalog that centralizes information to efficiently deliver knowledge, leading users from experimental design to publication.

Contents

This cytometrist’s companion guide is a combination of original material, hosted information, and external references, totaling more than 940 documents and links. A convenient tool perfect for continuing education, time-saving guidance, or exploration into new applications.

Latest Entries
– New Quick References on Pulse Processing & Area Scaling, Central Tendency Metrics for Fluorescence Data, and Index Sorting
– Links to the OLS Ontology Lookup Service, a repository for biomedical ontologies, and to the Human Cell Atlas, a resource under development aiming at providing comprehensive reference maps of all human cells (Workflow > Panel Design > Cell References & CD Charts)
– A link to the “Tandem Fluorophores” list by StepUpCytometry (Workflow > Panel Design > Spectra Viewers & Fluorescent Dyes)
– A couple of articles on spectral profile alterations when using different bead types or beads instead of cells (Workflow > Panel Design > Spectral Considerations)
– New commercial software and freeware (Workflow > Data Analysis > Software)
– An article on “Cleanet: Robust Doublet Detection in Cytometry Data Based on Protein Expression Patterns”, a link to Cytometry in R, an ongoing weekly mini-course organized by the University of Maryland’s Flow Cytometry Shared Service, a link to Fiji, an open-source image processing package, and a link to Honeychrome, an open-source GUI app for cytometry acquisition and analysis (Workflow > Data Analysis > Advanced Analysis Tools)
– New reagent compatibility issues (Workflow > Troubleshooting > Reagent Incompatibility)
– A link to “Data visualization: A view of every Points of View Column” featuring insights from Nature Methods Points of View articles on scientific figure design, visual communication, and data presentation (Workflow > Publishing)
– User guides for BD Biosciences FACSDiscover A8 and Cytek Biosciences Aurora Evo (Tech Corner > Instrumentation)
– A new Specialty Topic section on “Aquatic Research” with several articles on best practices and usage of both conventional flow cytometry and flow imaging microscopy, with links to the World Register of Marine Species, SeaDataNet, and AlgaeBase.
– Upcoming 2026 events from international flow cytometry associations (Industry Providers)
– Addition of several new vendors to the Providers List (Industry Providers)

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FlowPublicTM is a free repository of validated technical cytometry and computer resources.

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